Table 1. Molecular characteristics of Enterobacteriaceae isolates from wild urban brown rats, Vienna, Austria, 13 September 2016–6 June 2017 (n = 10).
| Isolatea | Species | Resistance profile | Serogenotyping | Phylogroup | ST | Antimicrobial resistance genes | Virulence factors | CTX environment | Repli-con | Transconjugants | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| O-serotyping | H-serotyping | β-lactamase | Non-β-lactamase | Mutation in gyrA | Mutation in parC | ISEcp1 5´ | ISEcp1 UP | tnpA IS26 | ||||||||
| AD8E | Escherichia coli | AMP, CFZ, CTX, FQR | 21 | 21 | B1 | ST101 | bla CMY-2, bla TEM-1 | NA | 83Ser-Leu, 87Asp-Asn | 80Ser-Ile | hemL, iss | NA | NA | NA | NA | NA |
| AD10Ea | E. coli | AMP, CFZ, CTX, FQR, TET, SXT | ND | 33 | C | ST34 | bla CTX-M-15, bla TEM-1 | aadA1, dfrA1, qrnS, sul2, tet(A) | 83Ser-Val | Wild type | hemL, intI1, mchB, mchC, mchF, pic, sat, sepA | 1.7 | 0.25 | NA | NA | NA |
| AD10Eb | E. coli | AMP, CFZ, CTX, TET, SXT | 86 | 18 | E | ST38 | bla CTX-M-9 | strB, sul2 | Wild type | Wild type | eatA, hemL, iss, nfaE | NA | NA | NA | NA | NA |
| AD19E | E. coli | AMP, CFZ, CTX | 6 | 49 | B1 | ST1079 | bla CTX-M-3 | NA | NA | NA | NA | NA | 0.25 | NA | NA | NA |
| AD21E | E. coli | AMP, CFZ, CTX | 6 | 49 | B1 | ST1079 | bla CTX-M-3 | NA | NA | NA | NA | NA | 0.25 | F | bla CTX-M-3 | |
| AD24E | E. coli | AMP, CFZ, CTX, SXT | 8 | 25 | B1 | ST58 | bla CTX-M-9, bla TEM-176 | dfrA5, sul2, strB | NA | NA | prfB, ireA, iss | NA | NA | NA | NA | NA |
| AD32E | E. coli | AMP, CFZ, CTX, ATM, FQR, TET, CHL, SXT | 9 | 30 | C | ST361 | bla CTX-M-15, bla TEM-176 | aadA4, aphA, dfrA17,strB, catA1, sul2, tet(B) | 83Ser-Leu, 87Asp-Asn | 80Ser-Ile | iss | 2.5 | 0.25 | NA | FIB, F | bla CTX-M-15, bla TEM-176 |
| AD54E | Enterobacter xiangfangensis | AMP, CFZ, CTX, FEP, IPM, MEM, ETP, ATM, FQR, GEN, TOB, TET, CHL, SXT | NA | NA | NA | ST114 | bla NDM-1, bla CTX-M-15, bla TEM176, bla OXA-1 | aac(6')-Ib-cr, aadA1, aadA2, catA1, catB3, dfrA1, dfrA12, dfrA14, qrnB1, ramA, sul1 | 83Ser-Leu | NA | NA | 2.5 | 0.25 | NA | H12 | bla CTX-M-15, bla TEM-176, bla OXA-1, aac(6')-Ib, aadA1, aadA2, catA1, catB3, dfrA1, dfrA12, dfrA14, qrnB1, sul1 |
| AD72E | En. xiangfangensis | AMP, CFZ, CTX, FEP, IPM, MEM, ETP, ATM, FQR, GEN, TOB, TET, CHL, SXT | NA | NA | NA | ST114 | bla NDM-1, bla CTX-M-15, bla TEM176, bla OXA-1 | aac(6')-Ib-cr, aadA1, aadA2, catA1, catB3, dfrA1, dfrA12, dfrA14,qrnB1, ramA, sul1 | 83Ser-Leu | NA | NA | 2.5 | 0.25 | NA | H12 | bla CTX-M-15, bla TEM-176, bla OXA-1, aac(6')-Ib, aadA1, aadA2, blaCTX, blaTEM, blaOXA, catA1, catB3, dfrA1, dfrA12, dfrA14, qrnB1, sul1, |
| AD74E | E. coli | AMP, CFZ, CTX, ATM, FQR, TET, CHL, SXT | 8 | 30 | B1 | ST1431 | bla CTX-M-1, bla TEM-1 | aadA1,aadA2, aphA, tet(A), catA1, cmlA1, dfrA12, floR, sul2, sul3 | 83Ser-Leu, 87Asp-Asn | 80Ser-Ile | hemL, intI1, iss | > 3 | 3 | NA | N | bla CTX-M-1, bla TEM-1 |
AMP: ampicillin; ATM: aztreonam; CFZ: cefazolin; CHL: chloramphenicol; CTX: cefotaxime; ETP: ertapenem; FEP: cefepime; FQR: fluoroquinolone; GEN: gentamicin; IPM: imipenem; MEM: meropenem; NA: data not available; ND: not detected; ST: sequence type; SXT: trimethoprim-sulfamethoxazole; TET: tetracycline; TOB: tobramycin.
a Rat ID + E: Enterobacteriaceae isolate. When several isolates were identified, they were given a letter (a, b, etc).