Table 2. Resistance and virulence genetic profile of Staphylococcus spp. isolates from wild urban brown rats, Vienna, Austria, 13 September 2016–6 June 2017 (n = 44).
| Isolatea | Speciesb | No. isolates | dru | SCCmec | CC | Antimicrobial resistance profile | Virulence factors | |
|---|---|---|---|---|---|---|---|---|
| Phenotype | Genes detected | |||||||
| AD6 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD7 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD8 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD9 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD9b_MRSP | S. pseudintermedius | 1 | dt11av | V | NA | BLA, TET, ERY, CLI, AMK, GEN, FQR | mecA, blaZ, blaI, blaR, tet(K), tet(M), erm(B), aacA-aphD, aphA3, sat | eno, isdA, lukS (ST22 + ST45) |
| AD10 | S. fleurettii (2)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD10b_S.xylosus_mecC | S. xylosus c | 1 | NA | XI | NA | BLA | mecC, blaZ-SCCmec XI | NA |
| AD11 | S. fleurettii (5)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD13 | S. fleurettii (6)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD15 | S. fleurettii (7)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD16 | S. fleurettii | 3 | NA | Irregular | NA | BLA | mecA | ssl10/set4 |
| AD16 | S. fleurettii (11)c | 1 | NA | Irregular | NA | BLA | mecA | ssl10/set4 |
| AD17 | S. fleurettii | 3 | NA | Irregular | NA | BLA | mecA | ssl10/set4 |
| AD19 | S. sciuri | 2 | NA | Irregular | NA | BLA, ERY, TET | mecA, blaZ, blaI, blaR, tet(M), erm(B) | NA |
| AD23 | S. fleurettii | 3 | NA | Irregular | NA | BLA | mecA | ssl10/set4 |
| AD24 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD25 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD27 | S. fleurettii (8)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD28 | S. fleurettii (9)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD30 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD31 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD32 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD33 | S. fleurettii (10)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD34 | S. fleurettii | 1 | NA | Irregular | NA | BLA | mecA | isaB, isdA, lukS (ST22 + ST45) |
| AD35 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD36 | S. fleurettii (12)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD37 | S. fleurettii (13)c | 1 | NA | Irregular | NA | BLA | mecA | NA |
| AD37 | S. fleurettii (4)c | 1 | NA | Irregular | NA | BLA | mecA | hlIII |
| AD38 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD39 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD50 | S. sciuri | 2 | NA | Irregular | NA | BLA, ERY, TET | mecA, blaZ, blaI, blaR, tet(M), erm(B) | NA |
| AD52 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD59 | S. fleurettii | 1 | NA | Irregular | NA | BLA | mecA | isaB, isdA, lukS (ST22 + ST45), ssl10/set4 |
| AD61 | S. fleurettii (3)c | 1 | NA | Irregular | NA | BLA | mecA | ssl10/set4 |
| AD68_MRSE | S. epidermidis | 1 | dt10a | Pseudo SCCmec | NA | BLA, TET, ERY, CLI, AMK, GEN | mecA, blaZ, blaI, blaR, tet(K) erm(C), dfrA, aacA-aphD | NA |
| AD69a | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD69b | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD70 | S. fleurettii (1)c | 1 | NA | Irregular | NA | BLA | mecA | ebpS, isaB, isdA, lukS (ST22 + ST45) |
| AD70_MRSA | S. aureus c | 1 | dt11a | VT | CC398 | BLA, TET, ERY, CLI | mecA, blaZ, tet(K), tet(M), erm(A) | aur, bbp,cap5, capH5, capJ5, capK5, clfA, clfB, cna, edh, ebpS, emp, fib (MRSA 252), fnbA, fnbB, hl, hla, hlgA, lukF, lukS, hlgA, hsdSx-CC15, hysA1 (MRSA252 + RF122) and/or hysA2 (cons), hysA1 (MRSA252 + RF122) and/or hysA2 (COL + US300), hysA2 (COL + US300 + NCTC), icaA, icaC, icaD, isaB (MRSA 252) isdA, ImrP, lukX, lukY, map, sdrC, sdrD, setB1, setB2, stB3, setC, set6-var, set11/set2, ssl02/set7, ssl04/set9, ssl05/set3, ssl07/set1, ssl09/set5, ssl10/set4, sspA, sspB, sspP, vwb |
| AD71 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD72 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD74 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
| AD74_MRSH | S. haemolyticus | 1 | NA | V | NA | BLA, TET, ERY, CLI, GEN | mecA, blaZ, blaI, blaR, aacA-aphD, mph(C), msr(A), aadD, aphA3, sat, qacA | isdA, ImrP-variant sspP-variant |
| AD76 | S. fleurettii | 19 | NA | Irregular | NA | BLA | mecA | NA |
AMK: amikacin; BLA: β-lactams; CLI: clindamycin; ERY: erythromycin; FQR: fluoroquinolone; GEN: gentamicin; NA: data not available (absent, therefore not amplified, or not typeable); TET: tetracycline.
a Rat ID + isolate identification if several isolates were isolated from the same rat.
b Number in bracket refers to the isolate identification in Figure 2.
c SCCmec-typing via array was performed on these isolates.