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. 2019 Aug 7;7:146. doi: 10.3389/fcell.2019.00146

Figure 9.

Figure 9

Scheme of intra-Golgi transport in S. cerevisiaewithin the framework of the KARM. This scheme ensures maturation and progression of cargo domains. According to the main postulate of the KARM, to become separated, it is necessary to have initial fusion. Thus, fusion is the first event, and then fission (at another site) is the second event. The ER is magenta; artificial cargo is blue and indicated with the letter “C”; the cis-Golgi compartment is red; the trans Golgi compartment is green; the post-Golgi compartment is brownish; the exited cargo domain is surrounded with a red line. The situation that is shown here was formed after several rounds of cargo domain detachment from ERES, when most of the Golgi compartments already contain the artificial cargo. (A) The initial situation when most of the Golgi compartments contain cargo domains (blue). (B) Fusion of the cis-Golgi compartment (red) with ERES, and the trans-Golgi compartment (green) with the post-Golgi compartment (brownish). (C) Shift of the COPII coat (yellow) from the cargo domain (blue oval surrounded by a magenta ring). There is fission of the connections between the trans compartment (green) and the cargo domain, which is consumed by the post-Golgi compartment. (D) Detachment of the cis-Golgi compartment connected to two cargo domains from ERES (yellow). (E) Rotation of the cis-Golgi (for the sake of clarity). (F) Fusion between the trans-Golgi compartment and the cis-Golgi compartment connected to cargo domains. (G) Detachment after fission of the cis-Golgi compartment from the trans-Golgi compartment connected to cargo domains. (H) Fusion of the cis-Golgi compartment with ERES, where a new cargo domain covered with Sec31 is prepared. (I) Shift of the Sec31-coat (yellow) from the cargo. (J) Detachment of the cis-Golgi compartment connected with the new cargo domain from ERES, and fusion of the trans-Golgi part connected to cargo domains with the post-Golgi. (K) Detachment of the trans-Golgi compartment from the cargo domains. (L) The final stage when the cargo domain of interest is within the post-Golgi compartment. (Ja–c) The alternative consequence of the final events; namely, the cargo domains connected with the trans-Golgi compartment can be delivered to the post-Golgi one after another.