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. Author manuscript; available in PMC: 2019 Aug 14.
Published in final edited form as: Clin Cancer Res. 2012 May 1;18(13):3603–3615. doi: 10.1158/1078-0432.CCR-11-3321

Figure 1: MK-2206 inhibits NB cell proliferation and Akt phosphorylation.

Figure 1:

A: NB cell lines and control ARPE-19 cells were treated with MK-2206 for 48 hrs. Cell proliferation was evaluated. B: NB cell lines were treated with MK-2206 for 16 hrs, caspase 3/7 activity was evaluated. Bar, SD. C: SY5Y, NGP, BE2 and AS cells were treated with MK-2206 for 48 hrs, stained with propidium iodide. The percentage of cells in S-G2/M, G1 and SubG1 phases is shown. D: SY5Y, NGP, BE2 and AS cells were treated with 7.5μM of MK-2206 for 24 hrs, stained with DCF-DA. E: SY5Y, NGP, BE2 and AS cells were treated with MK-2206 for 2 hrs, total protein was extracted for analysis of phosphorylated-Akt (P-Akt), total Akt, phosphorylated S6 (P-S6) and total S6.