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. 2019 Aug 7;10:1794. doi: 10.3389/fimmu.2019.01794

Figure 1.

Figure 1

(A,B) Percentage of autoreactive DSG1-positive (CD19+DSG1+) B cells (A) and DSG3-positive B cells (CD19+DSG3+) (B) among total B cells (CD19+) collected in 14 healthy donors and 20 pemphigus patients at baseline. Median with interquartile range were compared using the Mann-Whitney t-test. (C) Intensity of gene expression in one-cell sorted DSG-positive B cells (in gray n = 191 cells) and non-autoreactive DSG-negative B cells (in white, n = 201 cells) from pemphigus patients. The color intensity in each sorted cell depended on the ΔCT value relative to GAPDH house-keeping gene expression. Non-detected genes in one-cell qPCR were excluded from the map. (D) Comparison of the baseline frequencies of cells expressing the IL-1β, IL-12p35, IL-23p19 genes, and the transcription factor IRF5 gene between DSG-positive autoreactive B cells (gray columns, n = 191 cells) and non-autoreactive DSG-negative B cells (white columns, n = 201 cells) from pemphigus patients. Frequencies were compared using the Fisher's exact test.