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. 2019 Aug 7;10:1888. doi: 10.3389/fimmu.2019.01888

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Copyright © 2019 Niu, Zhou, Su, Wang, Xu, Yi, Wu, Du and Ren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the expression of LAG3 and PD-1 on CD4 and CD8 T cells at the onset of sepsis. The peripheral blood of septic patients and controls were collected within 12 h after enrollment, and then stained for flow cytometry. The positive rate relative to that of the isotype control staining was determined. Fluorescence intensity was quantified by calculating MESF values as described in the procedures. The horizontal lines represent the mean levels. Scattergram (A) and (B) showed the expression of LAG3 and PD-1 on CD4 and CD8 Tcells, respectively. Representative flow cytometry pictures (C). P-values were calculated with a non-parametric 2-tailed Mann-Whitney U test.