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. 2019 Apr 7;15(9):1606–1619. doi: 10.1080/15548627.2019.1591672

Figure 1.

Figure 1.

Autophagy is upregulated and required during myoblast differentiation. Representative immunoblots (a) and quantitative analysis (b, c) of MAP1LC3B (LC3B-I, LC3B-II, as well as calculated LC3B-II:I ratio) ATG7, BECN1, and BNIP3 during myoblast differentiation. Representative immunoblots (d) and quantitative analysis (e, f) of LC3B-I, LC3B-II, and SQSTM1 in CTRL (Vehicle) and chloroquine (CQ) treated myoblasts during differentiation. Also shown are representative ACT, GAPDH, and ponceau stained loading control blots/membranes. Representative immunoblots (g) of ATG7, LC3B-I, and LC3B-II in SCR and shAtg7 myoblasts and myotubes. Representative immunoblot (h) and quantitative analysis (i) of MYOG in SCR and shAtg7 cells during differentiation. Also shown is a representative ACT loading control blot. Representative images (j) of myotube formation in SCR and shAtg7 cells during differentiation. Cells were stained with DAPI (blue) and MF20 (red) to visualize nuclei and MYH, respectively. Scale bar = 100 µm. Quantitative analysis of the differentiation index (k) and fusion index (l) in SCR and shAtg7 cells during differentiation. *p < 0.05 compared to D0 (within group). †p < 0.05 between groups at the same time point.