Figure 6.

The protein-selective effects of autophagy in ATG7^−/− and ATG5^−/− cells are not explained by genetic compensation. (a) Autophagy-dependent turnover rates (h⁻¹) of mitochondrial proteins from ATG7^−/− fibroblasts with and without evidence of proteasomal turnover (ubiquitinated [Ub] sites) [48]. Red lines indicate means; n  =  44 proteins with ubiquitinated sites, 167 without. (b) Autophagy-dependent turnover rates (h⁻¹) of mitochondrial proteins from ATG5^−/− fibroblasts with (n  =  45) and without (n  =  151) ubiquitinated sites, as in panel A. (c) Autophagy-dependent turnover rates (h⁻¹) of individual mitochondrial proteins from ATG7^−/− fibroblasts with (n  =  31) and without (n  =  180) degrons for mitochondrial proteases Lon and YME1L1. (d) Autophagy-dependent turnover rates (h⁻¹) of individual mitochondrial proteins from ATG5^−/− fibroblasts with and without protease degrons (n  =  30 proteins with degrons, 166 without). All comparisons of means in panels A through D are nonsignificant by Student t test.