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. 2019 Mar 4;15(9):1506–1522. doi: 10.1080/15548627.2019.1586250

Figure 4.

Figure 4.

TRAF6 interacts with LC3B. (a) SW480 or HCT116 cells were immunoprecipitated (IP) with IgG, TRAF6 or LC3B antibody, and the immunoprecipitates were analyzed by western blot. (b) Western blot analysis of lysates after immunoprecipitation from HEK293T cells transfected with FLAG-TRAF6 and GFP-LC3B. (c) Representative images showing colocalization of transfected FLAG-TRAF6 and RFP-LC3B in HCT116 cells, examined by confocal microscopy. (d) Schematic representation of TRAF6 and mutants. (e) Immunoprecipitation and immunoblot of cell lysates from HEK293T cells expressing GFP-LC3B and deletion mutants of TRAF6 with MYC-tag. (f) Schematic representation of the YISL motif in TRAF6. (g) Immunoprecipitation and immunoblot of cell lysates from HEK293T cells expressing GFP-LC3B and MYC-TRAF6 or MYC-TRAF6Y406A,L409A.