FIGURE 3.

Liver gene expression of fatty acids metabolism (A) and gene expression of cholesterol and bile acid metabolism (B) after a short-term high fat diet (HFD). Mice fed a control diet and delivery vehicle-skimmed milk in drinking water (CT), mice fed a HFD and skimmed milk in drinking water (HF), and mice fed a HFD and supplemented with a suspension of 5 × 10⁸ cfu/mouse/day of B. animalis IPLA R1 strain in skimmed milk (HF-B) added to the drinking water. Each gene codes for the same name of the corresponding enzyme except Acaca that codes for ACC (Acetyl-CoA carboxylase) and Nr1h3 that codes for LXR (liver X receptor). Data are expressed as the mean ± SEM. Values are expressed as relative units with the mean of CT mice values set at 1. Data with different superscript letters are significantly different at p < 0.05 according to One-way analysis of variance statistical analysis followed by Tukey post hoc.