FIGURE 4.

Impact of the EPS-producing B. animalis IPLA R1 strain after a short-term high fat diet (HFD) on cecal microbiota assessed by qPCR. Total bacteria (A), Bifidobacterium (B), B. animalis (C), Lactobacillus (D), Bacteroides-Prevotella (E), Roseburia (F), and A. muciniphila (G) in the cecal content of mice after 3 days of treatment. Mice fed a control diet and delivery vehicle-skimmed milk in drinking water (CT), mice fed a HFD and delivery vehicle-skimmed milk in drinking water (HF), and mice fed with HFD supplemented with a bacterial suspension in milk (5 × 10⁸ cfu/mouse/day) of B. animalis IPLA R1 strain (HF-B) added to the drinking water. Data are box and whiskers plots with minimum and maximum. Data with different superscript letters are significantly different at p < 0.05 according to One-way analysis of variance statistical analysis followed by Tukey post hoc.