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. 2019 Aug 7;10:1792. doi: 10.3389/fmicb.2019.01792

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Copyright © 2019 Schoeters and Van Dijck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of the C2H system. (A) When no bait protein is attached to the DBD no interaction is possible and no transcription will take place. (B) Represents a situation in which no prey is attached to the AD leading to no transcription. In (C) both a prey and bait are present, but they do not interact so no transcription happens. Situation (D) depicts the interaction between a prey and bait protein thus leading to recruitment of RNA polymerase II and subsequent transcription. A common problem is the auto-activation by bait proteins. It is thus always essential to test auto-activation using the setup seen in part B.