KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bad | Cell Signaling Technology | Cat# 9239; RRID: AB_2062127 |
| Bak | Cell Signaling Technology | Cat# 12105; RRID: AB_2716685 |
| Active Bax 6A7 | Thermo Fisher | Cat# 14-6997-81; RRID: AB_468388 |
| Bax | Proteintech Group | Cat# 50599-2-lg |
| Bcl-xL | Cell Signaling Technology | Cat# 2764; RRID: AB_2228008 |
| Bim | Cell Signaling Technology | Cat# 2819; RRID: AB_10692515 |
| c-IAP1 | Cell Signaling Technology | Cat# 7065; RRID: AB_10890862 |
| Casp-2 | Abcam | Cat# ab179520 |
| Casp-7 | Abcam | Cat# ab201959 |
| Casp-9 | Abcam | Cat# ab202068 |
| cGAS | Cell Signaling Technology | Cat# 15102; RRID: AB_2732795 |
| Cleaved casp-3 | Cell Signaling Technology | Cat# 9661; RRID: AB_2341188 |
| Cytochrome C | Abcam | Cat# ab110325; RRID: AB_10864775 |
| IFNAR2 | Millipore | Cat# MAB1155; RRID: AB_2122758 |
| IκBα | Cell Signaling Technology | Cat# 9242; RRID: AB_331623 |
| IRF1 | Cell Signaling Technology | Cat# 8478; RRID: AB_10949108 |
| IRF3 (phospho Ser386) | Abcam | Cat# ab76493; RRID: AB_1523836 |
| IRF3 (phospho Ser396) | Cell Signaling Technology | Cat# 4947; RRID: AB_823547 |
| IRF3 | Abcam | Cat# ab68481; RRID: AB_11155653 |
| IRF3 | Proteintech Group | Cat# 11312-1-AP; RRID: AB_2127004 |
| JNK2 | Cell Signaling Technology | Cat# 9258; RRID: AB_2141027 |
| Phospho-JNK | Cell Signaling Technology | Cat# 4668; RRID: AB_823588 |
| Murine IgG2a Isotype Control | Sigma-Aldrich | Cat# M5409; RRID: AB_1163691 |
| Mcl1 | Cell Signaling Technology | Cat# 4572; RRID: AB_2281980 |
| Phospho-CDK substrate motif | Cell Signaling Technology | Cat# 9477; RRID: AB_2714143 |
| PUMA | Cell Signaling Technology | Cat# 12450 |
| RNA pol II CTD S2ph | Abcam | Cat# ab5095; RRID: AB_304749 |
| SMAC/Diablo | Cell Signaling Technology | Cat# 15108 |
| STING | Cell Signaling Technology | Cat# 13647; RRID: AB_2732796 |
| TBK1 | Abcam | Cat# ab40676; RRID: AB_776632 |
| α-Tubulin | Sigma-Aldrich | Cat# T9026; RRID: AB_477593 |
| XIAP | Cell Signaling Technology | Cat# 2045; RRID: AB_2214866 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ABT-199 | Selleck Chemicals | Cat# S8048; CAS No. 1257044-40-8 |
| ABT-263 | Selleck Chemicals | Cat# S1001; CAS No. 923564-51-6 |
| 2'3'-cGAMP | InvivoGen | Cat# tlrl-nacga23; CAS No. 1441190-66-4 |
| Doxycycline hyclate | Sigma Aldrich | Cat# D9891; CAS No. 24390-14-5 |
| IFNβ | R & D Systems | Cat# 8499-IF-010/CF |
| Nec-1s | BioVision | Cat# 2263; CAS No. 852391-15-2 |
| Nocodazole | Sigma-Aldrich | Cat# M1404; CAS No. 31430-18-9 |
| Pyridone 6 | MedChemExpress | Cat# HY-14435; CAS No. 457081-03-7 |
| Paclitaxel (Taxol) | Calbiochem | Cat# 580555; CAS No. 33069-62-4 |
| proTAME | Boston Biochemicals | Cat# I-440 |
| RO-3306 | Sigma Aldrich | Cat# SML0569; CAS No. 872573-93-8 |
| S63845 | Selleck Chemicals | Cat# S8383; CAS No. 1799633-27-4 |
| SiR-DNA | Cyotskeleton | Cat# CY-SC007 |
| Thymidine | Sigma-Aldrich | Cat# T1895; CAS No. 50-89-5 |
| Triptolide | Invivogen | Cat# ant-tpl; CAS No. 38748-32-2 |
| Z-VAD-FMK (ZVAD) | EMD-Millipore | Cat# 627610; CAS No. 187389-52-2 |
| Critical Commercial Assays | ||
| RNeasy Mini Kit | QIAGEN | Cat# 74014 |
| Transcriptor First Strand cDNA Synthesis kit | Roche | Cat# 04379012001 |
| LiqhtCycler 480 SYBR Green I Master mix | Roche | Cat# 04707516001 |
| Incucyte Caspase-3/7 Reagent | Essen Bioscience | Cat# 4440 |
| Experimental Models: Cell Lines | ||
| ARPE-19 hTERT | T. de Lange | N/A |
| BJ hTERT | T. de Lange | N/A |
| BT474 | K. Birsoy | RRID: CVCL_0179 |
| BT549 | K. Birsoy | RRID: CVCL_1092 |
| CAL51 | K. Birsoy | RRID: CVCL_1110 |
| HBL-100 | K. Birsoy | RRID: CVCL_4362 |
| HCC1143 | K. Birsoy | RRID: CVCL_1245 |
| HeLa CCL-2 | I Nakagawa | RRID: CVCL_0030 |
| HeLa Flp-In T-Rex | A. Desai/R. Gassmann | N/A |
| MDA-MB-157 | K. Birsoy | RRID: CVCL_0618 |
| MDA-MB-231 | K. Birsoy | RRID: CVCL_0062 |
| RUES2 | A. Brivanlou | human ESC registry no. 0013; RRID: CVCL_VM29 |
| T47D | K. Birsoy | RRID: CVCL_0553 |
| Experimental Models: Organisms/Strains | ||
| Immunodeficient mice, NOD-scid IL2Rgnull (NSG), male | Jackson Laboratory | Cat# 005557 |
| Xenopus laevis frogs, female | NASCO | Cat# LM00535M |
| Oligonucleotides | ||
| cGAS siRNA ON-TARGET plus SMARTpool | Dharmacon | Cat# L-015607-02 |
| Non-targeting control siRNA | Dharmacon | Cat# D-001810-01 |
| sgRNAs, see Table S2 | This paper | N/A |
| QPCR primers, see Table S2 | (Wu et al., 2013; Wu et al., 2018; Yoney et al., 2018) | N/A |
| Recombinant DNA | ||
| pcDNA5/FRT/TO | ThermoFisher | Cat# V652020 |
| pX330-U6-Chimeric_BB-CBh-hSpCas9 | (Cong et al., 2013) | Addgene Cat# 42230 |
| cGAS bacterial expression plasmids | This paper | N/A |
| Software and Algorithms | ||
| CellCognition (v. 1.5.2) | (Held et al., 2010) | http://www.cellcognition-project.org/ |
| Graphpad Prism (v.5.0a) | GraphPad Software | https://www.graphpad.com/ |
| ImageJ (v. 2.0.0-rc-43/1.51k) | NIH | https://imagej.nih.gov/ij/index.html |
| SoftWoRx | Applied Precision | N/A |
| Vision4D | Arivis | https://www.arivis.com/en/imaging-science/arivis-vision4d |