Figure 3.

Base pairing at the 3ʹ-end of crRNA with target RNA is important for RNA cleavage and binding of the Cmr-α complex in vitro.

(a) Schematic representation of two species of Cmr-α complexes (with 40/46 nt crRNAs) and RNA substrates (including 40 nt SS1 target RNA and different truncations) used in the cleavage assay. Red (for RNP complexes with 40/46 nt crRNAs) and black (only for RNP complexes with 46 nt crRNAs) triangles show the cleavage sites detected below. (b) RNA cleavage assay of 5ʹ-end labelled and 3ʹ-end labelled substrates (shown in A) by Cmr-α complexes for the indicated time points. (c) Target RNA binding assay of Cmr-α complexes from 0 nM to 100 nM with different lengths of target RNA, reactions were incubated with the same conditions as in (B).