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. 2019 Jun 24;16(9):1286–1299. doi: 10.1080/15476286.2019.1629208

Figure 6.

Figure 6.

Strand-specific mutations to disrupt hnRNP C1/C2 interaction with viral RNAs. (a) Schematic of U to C mutation generated at 569 position in 5ʹUTR. (b) UV-crosslinking assay was carried out using wild type or mutant radiolabelled stem-loop V with rhnRNP C1 or rPTB proteins. PTB and hnRNP C1 bands are indicated by arrows. Densitometry values of band intensities are indicated under the lanes. (c) Schematic of UU to CC mutation generated at fourth and fifth nucleotide position (from 3ʹend) in the negative-strand RNA. (d) UV-crosslinking gel image representing the interaction of recombinant hnRNP C1 to wild type and 4UU to CC mutant negative-strand RNAs. 1–100 nucleotide antisense RNA probe was used for this crosslinking. (e) Positive to negative-strand RNA ratio at 8-h post-transfection of wild type or 569U to C mutant CVB3 replicon RNA. * indicates P< 0.05.