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. 2019 Jun 24;16(9):1205–1214. doi: 10.1080/15476286.2019.1621622

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — RNA half-life determination. Northern analysis was performed in order to determine the effect of crhR mutation on the stability of selected RNA transcripts whose abundance dramatically changed in the microarray analysis. RNA half-lives were determined from total RNA (3 µg) isolated from wild type and crhR_TR cells for: (A) the mRNA crhR, (B) the sRNA Ncl1330, (C) the sRNA PmgR1 (Ncr0700) and (D) the sRNA SyR11/Ncr1160. 5S rRNA or rnpB abundance was utilized as a loading control for normalization. All experiments were performed in biological duplicates.