Figure 3.

Argonaute undergoes a conformational change as required by the speed-stability paradox.

(a) Cartoon representation of hAgo2-miRNA complex based on the wild-type hAgo2 structure (PDB ID: 4OLA). The different domains of hAgo2 (grey) are indicated. The miRNA guide is indicated in red, while the helix-7 fragment is indicated in yellow [66]. (B) Schematic drawing of miRNA, which is divided into five regions: the 5ʹ end anchored in a MID binding pocket, the Seed region (nt 2–8), the Mid-region where cleavage occurs on the opposing strand between nucleotide 10 and 11, the Supplementary region which supplements the binding of the seed domain. Lastly, the PAZ domain anchors the 3ʹ end of the guide [66]. Permission has been obtained for the above figures. Copyright 2017 John Wiley and Sons.