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The Lrh preQ1-II riboswitch senses the preQ1 concentration to yield a compact fold. The fraction of the riboswitch in a compact conformation, determined by the area under the 0.7 FRET peak in Fig (2a-f), was plotted as a function of preQ1 concentration and fitted to a hyperbola (red line; y = A*[preQ1]/(EC50+[preQ1])+ B, where EC50 is the half maximal effective concentration of preQ1 and B is the fraction of 0.7 FRET observed in the absence of preQ1).
