| Antibodies |
| NQO1 |
CST |
Cat#62262 |
| TXNRD1 |
CST |
Cat#15140 |
| GPX2 |
R&D |
Cat#MAB5470 |
| NRF2 |
R&D |
Cat#MAB3925 |
| FN3K |
Invitrogen |
Cat#PA5–28603 |
| EGFR |
CST |
Cat#4267 |
| phosphoERK |
CST |
Cat#4370 |
| tERK |
CST |
Cat#9102 |
| KEAP1 |
CST |
Cat#8047 |
| MAFG |
R&D |
Cat#MAB3924 |
| LDHA |
CST |
Cat#3582 |
| Histone H3 |
Abcam |
Cat#ab1791 |
| Lamin B |
Santa Cruz |
Cat#sc-374015 |
| β-actin |
Sigma-Aldrich |
Cat#A5441 |
| Tubulin |
Sigma-Aldrich |
Cat#T5168 |
| GAPDH |
CST |
Cat#2118 |
| Goat anti-rabbit-IR800 |
LI-COR |
Cat#926–32211 |
| Goat anti-mouse-IR680 |
LI-COR |
Cat#926–68070 |
| Donkey anti-goat-IR800 |
LI-COR |
Cat#925–32214 |
| Quick western IR680 |
LI-COR |
Cat#926–68100 |
| goat anti-mouse-HRP |
Santa Cruz |
Cat#sc-2005 |
| PE-conjugated NRF2 |
CST |
Cat#14409 |
| ChIP/IP NRF2 antibody |
CST |
Cat#14596 |
| IgG |
CST |
Cat#2729 |
| IHC: Ki67 |
Abcam |
Cat#ab16667 |
| IHC: HNF4a |
Santa Cruz |
Cat#sc-6556 |
| IHC: MYC |
Abcam |
Cat#ab32072 |
|
| HUMAN qPCR Probes |
| NQO1 |
Life Technologies |
Hs01045993_g1 |
| TXNRD1 |
Life Technologies |
Hs00917067_m1 |
| GPX2 |
Life Technologies |
Hs01591589_m1 |
| GCLC |
Life Technologies |
Hs00155249_m1 |
| FN3K |
Life Technologies |
Hs00223368_m1 |
| NRF2 |
Life Technologies |
Hs00975961_g1 |
| GUSB |
Life Technologies |
4333767 |
| ACTB |
Life Technologies |
4331182 |
| GAPDH |
Life Technologies |
4332649 |
| MOUSE qPCR Probes |
| Nrf2 |
Life Technologies |
Mm00477784_m1 |
| Actb |
Life Technologies |
Mm00607939_s1 |
|
|
|
| Bacterial and Virus Strains |
| Stable Competent cells |
NEB |
Cat#C3040H |
|
| Chemicals, Peptides, and Recombinant Proteins |
| Recombinant human NRF2 |
Origene |
Cat#TP760529 |
| Erlotinib |
Selleck Chemicals |
Cat#S1023 |
| MG-132 |
Sigma-Aldrich |
Cat#M7449 |
| Trametinib |
Selleck Chemicals |
Cat#S2673 |
| NAC |
Sigma-Aldrich |
Cat#A7250 |
| BSO |
Sigma-Aldrich |
Cat#B2640 |
| Ganciclovir |
Sigma-Aldrich |
Cat#G2536 |
|
| Critical Commercial Assays |
| CellTiter-Glo reagent |
Promega |
Cat#G9242 |
| Nano-Glo assay |
Promega |
Cat#N1110 |
| GSH/GSSG-Glo assay |
Promega |
Cat#V6611 |
| Glucose uptake-Glo assay |
Promega |
Cat#J1342 |
| T7 assay |
NEB |
Cat#M0302 |
| Genomic DNA |
Promega |
Cat#A1120 |
| Mini-Column Cellufine PB Affinity Chromatography |
AMS biotechnology |
Cat#202–51 |
| Oxidative stress Array |
Qiagen |
Cat#330231 |
| FAST Taqman Mastermix |
Applied Biosystems |
Cat#4444557 |
| FAST Sybr Green Mastermix |
Applied Biosystems |
Cat#4385612 |
| Fix/Perm Buffer |
Invitrogen |
Cat#00–5523-00 |
| NE-PER Kit |
Thermo Scientific |
Cat#78833 |
| H & E and IHC slides |
Histowiz.com |
N/A |
|
| Experimental Models: Cell Lines |
| H3255 |
ATCC |
Cat#CRL-2882; RRID: CVCL_0262 |
| H460 |
Lowe lab |
RRID: CVCL_0459 |
| HepG2 |
ATCC |
Cat#HB-8065; RRID: CVCL_0027 |
| 293T |
ATCC |
Cat#CRL-3216; RRID: CVCL_0063 |
| FL5–12 |
(McKearn et al., 1985) |
RRID: CVCL_0262 |
| Huh1 |
Tschaharganeh lab |
RRID: CVCL_02956 |
| PC9 |
Lowe lab |
RRID: CVCL_B260 |
|
MYC/sgKeap1 mHCC line |
This study |
N/A |
|
| Experimental Models: Organisms/Strains |
| C57BL/6J |
Jackson Laboratory |
Stock # 000664; RRID: IMSR_JAX000664 |
| Nude |
Jackson Laboratory |
Stock # 007850; RRID: IMSR_JAX007850 |
| NOD/SCID/IL2Rγ−/− (NSG) |
Jackson Laboratory |
Stock # 005557; RRID: IMSR_JAX005557 |
|
| Oligonucleotides |
| List of oligos |
See Table S7
|
N/A |
|
| Recombinant DNA |
| pNLF1-NRF2 (NRF2-nanoluc fusion) |
Promega |
N1391 |
| psPAX2 |
Trono lab |
Addgene # 12260 |
| psVSV.G |
Wendel lab |
N/A |
| LentiCRISPR V2 |
(Sanjana et al., 2014) |
Addgene # 52961 |
| pKLV Lentiviral backbone |
(Koike-Yusa et al., 2014) |
Addgene # 62348 |
| sgRNA library |
(Koike-Yusa et al., 2014) |
Addgene # 50947 |
|
| Software and Algorithms |
| Prism V7 |
GraphPad software |
https://www.graphpad.com |
| DAVID |
N/A |
https://david.ncifcrf.gov |
| cBioPortal |
N/A |
www.cBioPortal.org |
| Image studio Lite |
Li-COR |
https://www.licor.com/bio/image-studio-lite/ |
| Scaffold 4.8.4 |
N/A |
www.proteomesoftware.com/products/scaffold/ |
| ExPASy |
N/A |
www.expasy.org |
| R Package DESeq |
N/A |
https://www.huber.embl.de/users/anders/DESeq/ |
|
| Deposited Data |
| RNA sequencing data |
This study |
GEO accession GSE133160
|
| Proteomic data |
This study |
ProteomeXchange ID PXD014334
|
|
| Other Reagents |
| Magnetic protein A/G beads |
Life Technologies |
Cat#88803 |
| Purecol Collagen |
Fisher Scientific |
Cat#50360230 |
| MuMLV-RT |
NEB |
Cat#M0253 |
| RPMI 1650 |
MSKCC Media Core |
N/A |
| DMEM-HG |
MSKCC Media Core |
N/A |
| DMEM w/o glucose |
Life Technologies |
Cat#11966025 |
| Glucose |
Life Technologies |
Cat#A249001 |
| FBS |
Sigma-Aldrich |
Cat#F8317 |
| IL3 |
PeproTech, Inc |
Cat#213–13 |
| Puromycin |
Life Technologies |
Cat#A1113803 |
| Polybrene |
Sigma Aldrich |
Cat#TR-1003 |
| EGF |
Sigma Aldrich |
Cat#E9644 |
| TGFα |
PeproTech, Inc |
Cat#100–16A |
