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. 2019 Aug 14;14(8):e0216024. doi: 10.1371/journal.pone.0216024

Fig 1. Oleocanthal induces rapid necrotic cell death in a variety of cancer cells.

Fig 1

(A) The indicated cell lines were treated with 20 μM oleocanthal (OC) for 24 hours and viability was measured via the reduction of XTT. ****P < 0.0001 (One-way ANOVA). (B) PC3 cells were treated with 20μM oleocanthal or DMSO control for either 24 hours without media change, or 1 hour followed by a media change into full growth medium. Viability was measured 24 hours post treatment via the reduction of XTT. C and D) MDA-MB-231 cells (C) and PC3 cells (D) were treated with vehicle only (DMSO), or 20 μM oleocanthal for the indicated time points, and double-stained with Annexin-V FITC and PI. Fluorescence was measured on a flow cytometer (MoxiGo II). Treatment with 1μM Staurosporine (St) for 4 hours is presented as a positive control for apoptotic cells. Representative scatter plots from 3 independent experiments are shown, as well as bar graph quantifications: the lower right quadrant (apoptosis) is shown in green, and upper quadrant (necrosis) is shown in red. Bar graphs represent the mean ± SEM (n = 3).