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. 2019 Aug 14;14(8):e0217532. doi: 10.1371/journal.pone.0217532

Fig 6. HBB expression is variegated in transgenic mice carrying a deletion of the δβ intergenic promoter.

Fig 6

RNA FISH analysis of HBB transcripts in adult erythroid cells; primary and mature HBB transcripts are detected in green by an exon probe; as an internal control, Hbb-b1 primary transcripts are detected in red to identify erythroid cells; scale bar, 5 μm. Greater than 95% of FX14 erythroid cells have nuclear primary HBB signals and cytoplasmic HBB mRNA staining (top row). Conversely, the majority of FX115 and Δ115 cells lack primary HBB signals and do not contain cytoplasmic HBB mRNA (cells on the left). The few FX115 and Δ115 cells that do express HBB mRNA in the cytoplasm also have nuclear HBB primary transcript signals and vice-versa (cells on the right) showing that HBB expression is restricted to a small subset of adult erythroid cells.