Fig 7. The assembly and activity of the mitochondrial complexes I and V are compromised in the emp21-1 mutant.
(A) Polyacrylamide gel electrophoresis (BN-PAGE) analysis of the assembly of complex I and supercomplex I +III2. The gel was stained with Coomassie Brilliant Blue (CBB). (B) In gel assay of the NADH dehydrogenase activity displayed by complex I. Dihydrolipoamide dehydrogenase (DLDH) activity was used as the loading control. (C) In-gel assay for F1Fo-ATP hydrolyse activity of complex V. (D, E) Western blot analysis based on antibodies recognizing Atp1 (complex V) and Cytc1 (complex III). (F) Western blot analysis with antibody against Nad9, Cytc1, Cox2, Atp1 and ZmAOX. A gel stained with CBB was used for loading control. C-I: complex I, C-I+III2: supercomplex I +III2, C-V: complex V.