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. 2019 Aug 2;15(8):e1008305. doi: 10.1371/journal.pgen.1008305

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© 2019 Wang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 10 — (A) A yeast two hybrid (Y2H) assay identifies interactions among EMP21, EMP5 and ZmMORF8. The colony pictures were taken after three days incubation at 30°C in SD/-Trp-Leu dropout (DDO) plates, as well as six days incubation at 30°C in SD/-Trp-Leu-His-Ade dropout (QDO) plates. (B) A BIFC assay identifies interactions between EMP21 and ZmMORF8, as well as between EMP5 and ZmMORF8. Florescence signals observed by laser confocal microscopy ZEISS LSM 880. Mitochondria stained using MitoTracker Red. DIC: differential interference contrast. Bar = 10 μm.