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. 2019 Aug 8;4(15):e127569. doi: 10.1172/jci.insight.127569

Figure 2. S. epidermidis skin colonization does not change the composition or costimulatory molecule expression of LN APCs relative to GF APCs.

Figure 2

APCs from the peripheral LNs of specific pathogen–free (SPF), GF, and 10-day-painted S. epidermidis–monocolonized GF mice were analyzed by flow cytometry. (A) Gating strategy for different dendritic cell (DC) populations and fold change relative to the average in the percentage of DC subsets observed in GF controls within each experiment. LC, Langerhans cell. (B) Fold change in mean fluorescence intensity (MFI) relative to that in GF mice for costimulatory molecules on CD11c+-gated cells. Data were normalized from 3 individual experiments with n = 2–5 per group. Data represent the mean ± SEM. Statistical analyses were performed using the Kruskal-Wallis test for multiple comparisons. *P < 0.05; **P < 0.01.