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. 2019 Aug 8;4(15):e128722. doi: 10.1172/jci.insight.128722

Figure 2. Endothelial-specific deletion of Hsp47 in the heart.

Figure 2

(A) Schematic of breeding tamoxifen-inducible Tie2-CreERT2–transgenic mice with Hsp47-loxP–targeted mice. (B) Experimental regimen for mice subjected to TAC or a sham procedure for 4 weeks. Mice were injected at 6 weeks of age 2 times with tamoxifen and then put on tamoxifen chow before 8 weeks of age through harvesting at 12 weeks. (C) Western blot analysis for Hsp47 from endothelial cells isolated by FACS from the genotypes shown. Gapdh is a loading control. (D–G) Immunohistochemical heart images stained (scale bar: 100 μm) and quantified for collagen type I, III, and V from Tie2-CreERT2–transgenic mice and Hsp47 endothelial-specific deleted mice. Mice were subjected to TAC as shown in B. Quantitation shows mean intensity of immunoreactivity taken from 10 random histological sections from 5–10 mice in each group. *P < 0.05 versus Tie2-CreERT2 Sham. #P < 0.05 versus Tie2-CreERT2 TAC. P values were calculated with 1-way ANOVA with Tukey’s post hoc test.