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. 2019 Aug 8;4(15):e127823. doi: 10.1172/jci.insight.127823

Figure 3. Fatty acid synthase is reduced in the lungs of mice receiving high-fat diet and further reduced with hyperoxia.

Figure 3

(A) Differential lung expression by RNA-Seq of genes associated with mitochondrial function as determined by per mouse MitoCarta (an inventory of genes encoding proteins with mitochondrial localization) that were significantly different between the 60% and 10% fat diet mice. (B) Relative gene expression levels of fatty acid synthase (Fasn) from RNA-Seq of whole-lung homogenates from mice fed 60% and 10% fat diet exposed for 48 hours to hyperoxia or room air (n = 3 mice per group; the fold change of fragments per kilobase of transcript per million (FPKM) fragments sequenced was calculated relative to control, Benjamini-Hochberg method, *adjusted P < 0.05). (C) Western blot analysis for FASN in lungs with β-actin loading control. Densitometry analysis shown on the bottom (n = 2 per group for room air and n = 5 per group for hyperoxia, Mann-Whitney U test, *P < 0.05). (D) Representative section of FASN-stained lungs (n = 2 per group for room air and n = 7 per group for hyperoxia; original magnification, ×40). Data are expressed as mean ± SEM.