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. 2019 Aug 8;4(15):e127098. doi: 10.1172/jci.insight.127098

Figure 4. Prohibitin exhibits largely mitochondrial expression in Wilms’ tumor cell lines.

Figure 4

(A) Our in vitro studies of PHB included a control fibroblast cell line (BJ) as well as renal tumor cell lines (WiT49, WT-CLS1, and CCG9911). Endogenous PHB expression in the renal tumor cell line is shown with actin as a loading control. (B) PHB expression is compared in the different cell lines normalized to actin in Western blot triplicates. (C) Confocal fluorescence microscopy demonstrates that most cellular PHB (green) colocalizes with the inner mitochondrial membrane marker CoxIV (red) but not the nuclear marker DAPI (blue) in paraformaldehyde-fixed cells.