Figure 8. Regional knockout of rs1690789 in primary human lung fibroblasts using CRISPR/Cas9 editing.

Three pairs of sgRNAs were applied to delete a DNA region of ~100 bp spanning rs1690789 (A). The expression of TGFB2 is downregulated in rs1690789 knockout lung fibroblast cells with qPCR quantification, n = 4 (B). The editing efficiency is examined to confirm the effect of CRISPR/Cas9 regional knockout, n = 4 (C). *p<0.05 compared to control by unpaired t test.