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. 2019 Aug 8;4(15):e126764. doi: 10.1172/jci.insight.126764

Figure 11. ORC-13661 blocks entry of neomycin-Texas Red into brn3c:gfp zebrafish lateral line hair cell bodies in vivo.

Figure 11

Time-lapse imaging of lateral line neuromasts of brn3c:gfp-transgenic zebrafish exposed to neomycin-Texas Red (neo-TR) with and without ORC-13661. Images were captured at 30-second intervals for 50 minutes immediately following neo-TR treatment at a concentration of 200 μM. In the top row of images, hair cells show a progressive accumulation of intracellular neo-TR (200 μM). Some cells are dying by 20 minutes. In the bottom row of images, hair cells are exposed to neo-TR (200 μM) and cotreatment with ORC-13661 (8.33 μM). At this concentration, cotreatment with ORC-13661 virtually eliminates red fluorescence in hair cell bodies and all cells survive. Times at the top of each panel indicate time (minutes) from introduction of neo-TR. Scale bar: 1 μm.