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. 2019 Aug 8;4(15):e126764. doi: 10.1172/jci.insight.126764

Figure 5. ORC-13661 protects mouse outer hair cells from cisplatin damage.

Figure 5

(A–C) Mouse cochlear cultures prepared from P2 mice were maintained in vitro for 1 day and then divided into 3 conditions for an additional 48 hours: low-serum medium (LSM) alone, LSM containing 5 μM cisplatin, or LSM containing 5 μM cisplatin in the presence of 20 μM ORC-13661. Hair cell loss was not seen in LSM alone (A), and substantial outer hair cell loss was seen in the presence of 5 μM cisplatin (B). Coincubation with 20 μM ORC-13661 offered full protection (C). I, inner hair cell row; O1, OHC row 1; O2, OHC row 2; O3, OHC row 3. (D) Dose-response function of ORC-13661 protection against 5 μM cisplatin. As in the gentamicin assay, concentrations of ORC-13661 ≤5 μM offered no protection, 10 μM offered partial protection, and ≥20 μM offered full protection. Results are shown for the basal region of interest. OHCs were counted in a 221-μm length of the organ of Corti from each cochlea. Numbers of cochleae counted were as follows: control 8; 5 μM cisplatin, 8; 5 μM cisplatin + [ORC-13661]: 3 μM, 6; 5 μM, 5; 10 μM, 7; 20 μM, 6; 30 μM, 5; 40 μM, 5. For statistical analysis 1-way ANOVA was used. Error bars represent SEM. Scale bar: 20 μm.