Fig. 5. Dox-CBD-SA treatment completely eradicates established MC38 tumor in combination with anti-PD-1 CPI.
MC38 cells (5 × 105) were inoculated on day 0. Mice were injected intravenously with aldoxorubicin or Dox-CBD-SA (5 mg/kg on a Dox basis) on days 6, 9, and 12. αPD-1 was also injected intraperitoneally on days 10 and 13. (A) The experimental schedule. (B) Graphs depict tumor volume until the first mouse died (mean ± SEM). (C) Survival rate. (D to G) Individual tumor growth curves. (H) On day 60, Dox-CBD-SA + αPD-1–treated survivors were rechallenged by subcutaneous injection of 5 × 105 MC38 cells. Naïve mice were also challenged with the same amounts of cells as a control group. The number of mice that developed palpable tumors is shown. Two experimental replicates. Statistical analyses were done using log-rank (Mantel-Cox) test for survival curves. *P < 0.05; **P < 0.01.