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. 2019 Aug 14;5(8):eaax1101. doi: 10.1126/sciadv.aax1101

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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 2 — (A) Schematic representation of the CRISPR-Cas9 genome editing system to the Zcwpw1-deficient mice. (B) Western blotting showed that ZCWPW1 was deleted in PD16 Zcwpw1^−/− testes compared to wild type. β-Actin was used as the loading control. (C and D) Co-immunoprecipitation analysis of ZCWPW1 and H3K4me3 from PD18 testis protein extracts. ZCWPW1 immunoprecipitated with H3K4me3. (E). Testes from PD35 Zcwpw1^+/+ and Zcwpw1^−/− male mice. Zcwpw1^−/− male mice had reduced testis size at PD35 (n = 3, Welch’s t test analysis; P < 0.0001). (F) Hematoxylin and eosin (H&E) staining of adult Zcwpw1^+/+ testis. (G) H&E staining of adult Zcwpw1^−/− testis sections showed complete arrest of spermatogenesis. Arrows, apoptotic spermatocytes; arrowhead, empty seminiferous tubules; asterisk, seminiferous tubules lack of postmeiotic spermatocytes. (H) Cumulative numbers of pups per female during the defined time period. n = 3 mice for each genotype. (I to N) Histological analysis of ovaries from Zcwpw1^+/+ and Zcwpw1^−/− females. (I and J). Morphological studies of ovaries showed that at 3-month Zcwpw1^−/− ovaries (J) exhibited similar morphologies as Zcwpw1^+/+ ovaries (I). (K and L). At 6 months of age, Zcwpw1^−/− ovaries (L) contained fewer but healthy follicles and corpora lutea (CL). (M and N). At around 8 months of age, the mutant ovaries (N) had no oocytes or follicles. (Photo credit: Miao Li and Tao Huang, Shandong Provincial Hospital Affiliated with Shandong University).