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. 2019 Aug 14;5(8):eaax1101. doi: 10.1126/sciadv.aax1101

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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 4 — (A to U) Immunostaining for SYCP3 (red) and recombination protein (green) was performed on Zcwpw1^+/+ and Zcwpw1^−/− spermatocytes from PD25 mice. Representative images of spermatocytes at the leptotene, zygotene, and pachytene (pachytene-like) stages are shown. Each dot represents the number of DNA repair protein foci per cell, with black dots indicating Zcwpw1^+/+ spermatocytes and red dots indicating Zcwpw1^−/− spermatocytes. Solid lines show the mean and SD of foci number in each group of spermatocytes. (A to G) RPA2 foci. (H to N) RAD51 foci. (O to U) DMC1 foci. P values were calculated by Student’s t test. (V to X) Immunostaining for SYCP3 (red) and TRF1 (green) was performed on Zcwpw1^+/+ and Zcwpw1^−/− spermatocytes. Representative images of spermatocytes at the pachytene (pachytene-like) stages are shown. Zcwpw1^−/− spermatocytes had comparable numbers of TRF1 foci on equator images as compared to Zcwpw1^+/+ spermatocytes. n.s., no statistical significance.