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. 2019 Aug 14;5(8):eaax1101. doi: 10.1126/sciadv.aax1101

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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

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Fig. 6 — (A and B) Representative Zcwpw1^+/+ (A) and Zcwpw1^−/− (B) ovary sections from E13.5 females immunostained for mouse vasa homolog (MVH) with hematoxylin counterstaining. (C) Oocyte counts (relative numbers) showed that there were similar numbers of oocytes in E13.5 Zcwpw1^+/+ and Zcwpw1^−/− females. MVH-positive cells were counted. (D and E) Representative Zcwpw1^+/+ (D) and Zcwpw1^−/− (E) ovary sections from PD1 females immunostained for MVH with hematoxylin counterstaining. (F) Relative oocyte counts showed that Zcwpw1^−/− ovaries contained significantly fewer oocytes than Zcwpw1^+/+ ovaries. (G and H) Representative Zcwpw1^+/+ (G) and Zcwpw1^−/− (H) ovary sections from PD8 females immunostained for MVH with hematoxylin counterstaining. (I) Relative oocyte counts showed that Zcwpw1^−/− ovaries contained significantly fewer follicles than Zcwpw1^+/+ ovaries. MVH-positive oocyte nuclei with characteristic surrounding granulosa cell layers were counted. In all cases, counts were made for every section (8 μm per section) and summed to calculate the total number of oocytes per ovary. For each genotype, six ovaries from three mice were analyzed. P values were calculated by Student’s t test.