Table 3.
Preliminary SAR of Representative Amidino-Rocaglates.a
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IC50 was determined from fitted sigmoid curves; IC50 indicates the concentration of compound that inhibits cap-dependent translation by 50%, which was normalized by cap-independent translation. For IC50 determination, the bicistronic reporter FF/HCV/Ren mRNA was used to produce Firefly luciferase (cap-dependent) and Renilla luciferase (cap-independent) in Krebs-2 extracts in the presence of indicated compounds at various concentrations.
