Table 1.
Primer sequences for real-time PCR
| Gene | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|
| GAPDH | TGACTCTACCCACGGCAAGTTCAA | ACGACATACTCAGCACCAGCATCA | 141 |
| ACE | GTGTTGTGGAACGAATACGC | CCTTCTTTATGATCCGCTTGA | 187 |
| AT1-R | CTCAAGCCTGTCTACGAAAATGAG | GTGAATGGTCCTTTGGTCGT | 188 |
| TNF-α | GCCGATTTGCCACTTCATAC | AAGTAGACCTGCCCGGACTC | 209 |
| IL-6 | GCCTATTGAAAATCTGCTCTGG | GGAAGTTGGGGTAGGAAGGA | 160 |
| IL-1β | AGCAACGACAAAATCCCT GT | GAAGACAAACCGCTTTTCCA | 209 |
| CD11b | TTACCGGACTGTGTGGACAA | AGTCTCCCACCACCAAAGTG | 239 |
ACE, angiotensin-converting enzyme 1; AT1-R, angiotensin II type 1 receptor; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; IL-6, interleukin-6; CD11b, cluster of differentiation molecule 11b.