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. 2019 Aug 14;9:11861. doi: 10.1038/s41598-019-47891-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of ER-INP promotes angiogenesis of chick chorioallantoic membranes (CAMs). Culture supernatant of cells transfected with ER-INP or the control vector was subjected to a CAM assay to determine the effect of ER-INP on angiogenesis. CAM blood vessels were observed in the entire field and photographed (A,D). Representative images of CAMs. Number of branches of maximum blood vessel were counted (B,E) and the summed area was measured by Image Pro Plus statistics software (C,F). Data represent the mean ± SD from 6 eggs for each group (B,C,E,F).