. 2019 Aug 14;9:11859. doi: 10.1038/s41598-019-48281-3

Table 1.

Effect of postovulatory aging on the pattern of Ca²⁺ oscillations and Ca²⁺ ER store in oocytes.

	fresh	9 hrs in vitro	25 hrs in vitro	9 hrs in vivo	25 hrs in vivo
No. of Ca²⁺ transients	7.0^a (6.0; 8.0) n = 64	8.0^f (7.0; 9.0) n = 57	8.0 (6.0; 10.0) n = 30	6.0^f,b (5.0; 7.5) n = 51	12.0^a,b (7.0; 19.0) n = 43
Duration of Ca²⁺ oscillations (min)	158.0^a (128.3; 182.2) n = 64	159.9^b (141.7; 171.8) n = 57	165.3^c (107,1; 209.0) n = 30	88.0^a,b,c,d (64.1; 114.0) n = 47	183.5^d (117.9; 279.5) n = 42
Mean interval between Ca²⁺ transients during 1^st 2 hrs (min)	23.4^a,b,f (20.5; 25.3) n = 64	19.6^f (16.2; 21.3) n = 57	20.7 (15.5; 26.0) n = 30	16.8^a (14.6; 19.0) n = 47	14 ^b (10.1; 19.9) n = 42
Amplitude of the 1^st Ca²⁺ transient (ΔF/F₀)	0.9^a,b,c (0.8; 1.0) n = 64	0.8^d,e (0.7; 0.9) n = 57	0.7^a (0.6; 0.8) n = 30	0.6^b,d (0.5; 0.7) n = 50	0.6^c,e (0.5; 0.6) n = 43
Amplitude of the 3^rd Ca²⁺ transient (ΔF/F₀)	0.8^a,b,c (0.8; 0.9) n = 64	0.8^d,h (0.7; 0.8) n = 57	0.7^a,h (0.6; 0.7) n = 30	0.7^b (0.6; 0.7) n = 45	0.6^c,d (0.5; 0.6) n = 41
Duration of the 1^st Ca²⁺ transient (min)	3.2^a,b,f (2.8; 3.5) n = 64	2.2^a,g (2.0; 2.5) n = 57	1.7^b,c,h (1.4; 2.0) n = 30	3.3^c,g (2.5; 4.7) n = 50	2.2^f,h (1.8; 3.0) n = 43
Duration of the 3^rd Ca²⁺ transient (min)	1.1 (1.0; 1.2) n = 64	1.2 (1.0; 1.2) n = 57	1.0 (0.8; 1.2) n = 30	1.2 (1.0; 1.2) n = 45	1.0 (0.8; 1.2) n = 41
Rate of the 1^st Ca²⁺ increase (min⁻¹)	3.1^a,b (2.4; 3.7) n = 64	3.9^c,d (3.1; 4.6) n = 57	3.0^h,f (2.3; 3.5) n = 30	2.2^a,c,h (1.4; 2.7) n = 49	2.0^b,d,f (1.6; 2.3) n = 43
Rate of the 3^rd Ca²⁺ increase (min⁻¹)	4.7^a,b,c (4.4; 5.2) n = 64	4.3^d,h (4.0; 4.6) n = 57	3.7^a,h (3.4; 4.0) n = 30	3.8^b (3.3; 4.2) n = 45	3.1^c,d (2.9; 3.3) n = 41
Rate of the 1^st Ca²⁺ decrease (min⁻¹)	−4.1^a,b,c (−4.4; −3.6) n = 64	−3.5^d,f (−3.9; −3.2) n = 57	−2.4^a,f (−3.4; −2.0) n = 30	−3.1^b (−3.4; −2.5) n = 49	−2.7^c,d (−3.1; −2.1) n = 43
Rate of the 3^rd Ca²⁺ decrease (min⁻¹)	−4.7^a,b,c (−5.2; −4.4) n = 64	−4.2^f,h (−4.5; −3.9) n = 57	−3.4^a,h (−3.8; −2.9) n = 30	−3.9^b (−4.2; −3.4) n = 45	−3.2^c,f (−3.7; −2.9) n = 41
Amplitude of the TG-induced Ca²⁺ release (ΔF/F₀)	1.6^a,b,c, (1.4; 1.7) n = 65	1.3^a,f (1.3; 1.4) n = 75	1.3^b,g (1.2; 1.4) n = 53	1.3^c,f,g (1.2; 1.3) n = 63	1.4^c (1.3; 1.4) n = 56

fresh

9 hrs in vitro

25 hrs in vitro

9 hrs in vivo

25 hrs in vivo

Median (Q1; Q3)

No. of Ca²⁺ transients

7.0^a

(6.0; 8.0)

n = 64

8.0^f

(7.0; 9.0)

n = 57

8.0

(6.0; 10.0)

n = 30

6.0^f,b

(5.0; 7.5)

n = 51

12.0^a,b

(7.0; 19.0)

n = 43

Duration of Ca²⁺ oscillations (min)

158.0^a

(128.3; 182.2)

n = 64

159.9^b

(141.7; 171.8)

n = 57

165.3^c

(107,1; 209.0)

n = 30

88.0^a,b,c,d

(64.1; 114.0)

n = 47

183.5^d

(117.9; 279.5)

n = 42

Mean interval between Ca²⁺ transients during 1^st 2 hrs (min)

23.4^a,b,f

(20.5; 25.3)

n = 64

19.6^f

(16.2; 21.3)

n = 57

20.7

(15.5; 26.0)

n = 30

16.8^a

(14.6; 19.0)

n = 47

14 ^b

(10.1; 19.9)

n = 42

Amplitude of the 1^st Ca²⁺ transient (ΔF/F₀)

0.9^a,b,c

(0.8; 1.0)

n = 64

0.8^d,e

(0.7; 0.9)

n = 57

0.7^a

(0.6; 0.8)

n = 30

0.6^b,d

(0.5; 0.7)

n = 50

0.6^c,e

(0.5; 0.6)

n = 43

Amplitude of the 3^rd Ca²⁺ transient (ΔF/F₀)

0.8^a,b,c

(0.8; 0.9)

n = 64

0.8^d,h

(0.7; 0.8)

n = 57

0.7^a,h

(0.6; 0.7)

n = 30

0.7^b

(0.6; 0.7)

n = 45

0.6^c,d

(0.5; 0.6)

n = 41

Duration of the 1^st

Ca²⁺ transient (min)

3.2^a,b,f

(2.8; 3.5)

n = 64

2.2^a,g

(2.0; 2.5)

n = 57

1.7^b,c,h

(1.4; 2.0)

n = 30

3.3^c,g

(2.5; 4.7)

n = 50

2.2^f,h

(1.8; 3.0)

n = 43

Duration of the 3^rd

Ca²⁺ transient (min)

1.1

(1.0; 1.2)

n = 64

1.2

(1.0; 1.2)

n = 57

1.0

(0.8; 1.2)

n = 30

1.2

(1.0; 1.2)

n = 45

1.0

(0.8; 1.2)

n = 41

Rate of the 1^st Ca²⁺ increase (min⁻¹)

3.1^a,b

(2.4; 3.7)

n = 64

3.9^c,d

(3.1; 4.6)

n = 57

3.0^h,f

(2.3; 3.5)

n = 30

2.2^a,c,h

(1.4; 2.7)

n = 49

2.0^b,d,f

(1.6; 2.3)

n = 43

Rate of the 3^rd Ca²⁺ increase (min⁻¹)

4.7^a,b,c

(4.4; 5.2)

n = 64

4.3^d,h

(4.0; 4.6)

n = 57

3.7^a,h

(3.4; 4.0)

n = 30

3.8^b

(3.3; 4.2)

n = 45

3.1^c,d

(2.9; 3.3)

n = 41

Rate of the 1^st Ca²⁺ decrease (min⁻¹)

−4.1^a,b,c

(−4.4; −3.6)

n = 64

−3.5^d,f

(−3.9; −3.2)

n = 57

−2.4^a,f

(−3.4; −2.0)

n = 30

−3.1^b

(−3.4; −2.5)

n = 49

−2.7^c,d

(−3.1; −2.1)

n = 43

Rate of the 3^rd Ca²⁺ decrease (min⁻¹)

−4.7^a,b,c

(−5.2; −4.4)

n = 64

−4.2^f,h

(−4.5; −3.9)

n = 57

−3.4^a,h

(−3.8; −2.9)

n = 30

−3.9^b

(−4.2; −3.4)

n = 45

−3.2^c,f

(−3.7; −2.9)

n = 41

Amplitude of the TG-induced Ca²⁺ release (ΔF/F₀)

1.6^a,b,c,

(1.4; 1.7)

n = 65

1.3^a,f

(1.3; 1.4)

n = 75

1.3^b,g

(1.2; 1.4)

n = 53

1.3^c,f,g

(1.2; 1.3)

n = 63

1.4^c

(1.3; 1.4)

n = 56

Values marked with the same letter are significantly different: ^a,b,c,d,ep < 0.001, ^f,gp < 0.01, ^hp < 0.05.