Table 1.

Primers and oligonucleotides used in this study

Amplicon Primers (1st PCR reaction)
Amplicon-TBC1D4-FWD-1	ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNCAAGGAAAATAAAGGGTCAAGTCAA
Amplicon-TBC1D4-REV-1	CTGGAGTTCAGACGTGTGCTCTTCCGATCCTTCCTCTTACTGGCTCTGCAG
Amplicon Primers (2nd PCR reaction)
Amplicon-3A-REV-2	CAAGCAGAAGACGGCATACGAGATCAACAAGTGACTGGAGTTCAGACGT
Amplicon-3A-ssODN-REV-2	CAAGCAGAAGACGGCATACGAGATGTTGTTGTGACTGGAGTTCAGACGT
T7 Endonuclease Primers
T7-TBC1D4-FWD	GGCCACCATACCATCTTCACA
T7-TBC1D4-REV	ATTTGGCTCTGCTTGTAGCC
RT-PCR 1 kb TBC1D4 amplicons
TBC1D4_1kb-1-FWD	GCTTTGTGCCAACTAGCATGT
TBC1D4_1kb-1-REV	ACTGGGTAACAGTGCGGAGG
TBC1D4_1kb-2-FWD	AGGGCCAATAGCCAACTGAA
TBC1D4_1kb-2-REV	TGGGTAGACTCAGCCACAATG
TBC1D4_1kb-3-FWD	TGGTGCATGTCAGAAAGAGGT
TBC1D4_1kb-3-REV	TCAGTTGGCTATTGGCCCTC
TBC1D4_1kb-4-FWD	TGAAGCCAAGCAGAAGACACA
TBC1D4_1kb-4-REV	TCCTCGTCGACTTTTGGGAA
TBC1D4_1kb-5-FWD	TTCCAGGTTGGGCGATTTGA
TBC1D4_1kb-5-REV	TCCCTTCTCCATCACTGCAC
4 kb fragment flanking target site
TBC1D4-4kb-FWD	CCTGGAAAATCTTAATGGTGCTTC
TBC1D4-4kb-REV	GCCTAATAAACAACAGCCTCCTG
TBC1D4 gRNA cloning oligonucleotides and ssODN template (editing)
TBC1D4 gRNA-edit-FWD	CACC ATTGGTATGGGACTTTCCTA*
TBC1D4 gRNA-edit-REV	AAAC TAGGAAAGTCCCATACCAAT
TBC1D4 ssODN Template	CTTGAGCTGGCAATGTGAGTCCTGCATCACTAAAAGGAGAGTTCTATACACAGAAACAAATCCGTCTTCACATCAAAGCTGTCTATATTGGTATGGGCATTTTTCTAGGGCCACAA AAATGAAGGGGGATGTTAGCTTCCTTGTGAAATGATTA CTCATGTCATTTAGAACTTGCAAGAGTGCCAGGTTTTAAAATGTTT

*Sequences in bold correspond to the targeted 20-nt gRNA