Table 2.

Conditions tested and efficiencies of transfections

Plasmida	Transfection conditionsb	Number of cells (million)	Plasmid (µg)	Plasmid (molecules)	Approximate transfection efficiency (microscopy, percent GFP + cells)
GFP	X-001	4	2	6.78E + 12	0
	T-020	4	2	6.78E + 12	10–15
	U-009	4	2	6.78E + 12	10–15
pCAG-eCas9-GFP-U6-gRNA	U-009	2	5	4.76E + 11	0
	U-009	4	10	9.51E + 11	1
	U-009	2	16.6	1.58E + 12	0
	U-009	4	33.3	3.17E + 12	2–5

^aAddGene 79145

^bAmaxa Nucleofector II programs