Fig. 3.

TAK-003-elicited CD8⁺ memory precursors exhibit a unique transcriptional profile. a Transcriptional heterogeneity of sorted CD38⁺HLA-DR⁺ CD8⁺ T cells isolated 14 days post-TAK-003 administration as assessed by single-cell RNA sequencing. Unsupervised cell clustering and data visualization were performed using sparse nearest-neighbour graphing, followed by Louvain Modularity Optimization. b Hierarchical heat map of differentially expressed genes within the sorted CD38⁺HLA-DR⁺ CD8 T cell. c Expression of select transcripts within the sorted CD38⁺HLA-DR⁺ CD8⁺ T cells. d Distribution of CD38⁺HLA-DR⁺ CD8⁺ T cells at day 14 with TCR clonotypes overlapping with NS1- and NS3-reactive memory CD8⁺ T cells at day 120 (memory precursors). e Distribution of clonally expanded CD38⁺HLA-DR⁺ CD8⁺ T cells at day 14