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. 2019 Aug 14;10:3666. doi: 10.1038/s41467-019-11634-7

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — In vitro activated T cells can be identified by changes in metabolite transporter expression and metabolite utilization. CD8⁺ T cells from healthy donors were analyzed by flow cytometry at the indicated time points after in vitro stimulation with 0.1 μg ml⁻¹ αCD3 and 1 μg ml⁻¹ αCD28. T cell activation was assessed based on expression of a, b CD69, CD25, c, d TfR1, CD98, and e, f 2-NBDG BODIPY FL-C16 uptake. Error bars show mean and SEM. Results are representative of two independent experiments with a total of four individual donors. Source data are provided as a Source Data file