Fig. 5.

Antigen-specific in vitro CD8⁺ T cell activation results in increased metabolite transporter expression and substrate utilization. a CD8⁺ T cells from healthy donors were analyzed by flow cytometry after 48 h in vitro stimulation with 1μg ml⁻¹ of the indicated peptide pool. T cell activation was assessed based on b upregulation of CD69 and CD25 expression, c increased uptake of BODIPY FL-C₁₆ and increased CD25 expression, d increased expression of CD98 and CD25, and e increased expression of TfR1 and CD25. Error bars show mean and SEM. The percentage of CD8 T cells exhibiting a metabolically activated state as assessed by BODIPY FL-C16 uptake, CD98 expression, and TfR1 expression following in vitro stimulation with f hCMV g adenovirus, or h influenza derived peptide stimulation was highly correlated with the percentage of cells expressing the conventional activation markers CD69 and CD25 at the same time point. Results are representative of two independent experiments with a total of 15 individual donors. Source data are provided as a Source Data file