Table 1.
Dominant TCR clonotypes from acutely activated and memory CD8+T cells
| Population | Day | Frequency | TCRα CDR3aa | TRAV | TRAJ | TCRβ CDR3aa | TRBV | TRBD | TRB J |
|---|---|---|---|---|---|---|---|---|---|
| CD8+CD38−HLA-DR− (resting) | 14 | 0.011847 | CAVMDSNYQLIW | TRAV1-2 | TRAJ33 | CASSEGAKNIQYF | TRBV6–4 | − | TRBJ2–4 |
| 0.009756 | CASMDSNYQLIW | TRAV1-2 | TRAJ33 | CATSDLVQGDTGELFF | TRBV24–1 | TRBD1 | TRBJ2-2 | ||
| 0.008362 | CAMNTDAGGTSYGKLTF | TRAV12-3 | TRAJ52 | CAIRQGNTEAFF | TRBV10-3 | TRBD1 | TRBJ1-1 | ||
| 0.006969 | CAVMDSNYQLIW | TRAV1-2 | TRAJ33 | CSARQGEYEQYF | TRBV20-1 | TRBD1 | TRBJ2-7 | ||
| 0.006272 | CGRRGPPTDKLIF | TRAV13-2 | TRAJ34 | CASSQGPTVGQPQHF | TRBV4-1 | TRBD1 | TRBJ1-5 | ||
| 0.005575 | CAVRDDRGEGTYKYIF | TRAV3 | TRAJ40 | CASSLSQGYQPQHF | TRBV7-6 | TRBD1 | TRBJ1-5 | ||
| 0.004878 | CAVMDSNYQLIW | TRAV1-2 | TRAJ33 | CSARGGLEVDTQYF | TRBV20-1 | TRBD1 | TRBJ2-3 | ||
| 0.004878 | CAVMDSNYQLIW | TRAV1-2 | TRAJ33 | CASSDSGATGELFF | TRBV6-4 | TRBD1 | TRBJ2-2 | ||
| 0.004878 | CAVKDSNYQLIW | TRAV1-2 | TRAJ33 | CSARTGTSVGSFSYEQYF | TRBV20-1 | TRBD2 | TRBJ2-7 | ||
| 0.004181 | CAVLDSNYQLIW | TRAV1-2 | TRAJ33 | CSARDLDRDNSPLHF | TRBV20-1 | TRBD1 | TRBJ1-6 | ||
| CD8+CD38+HLA-DR+ (activated) | 14 | 0.01271571 | CAVRGRGDYKLSF | TRAV1-2 | TRAJ20 | CASSSAGTLNTGELFF | TRBV7-9 | TRBD1 | TRBJ2-2 |
| 0.00999092 | CAGRGAGSYQLTF | TRAV21 | TRAJ28 | CASSLLSYEQYF | TRBV7-9 | − | TRBJ2-7 | ||
| 0.00726612 | CAVQAGGYSTLTF | TRAV20 | TRAJ11 | CASAEADNEQFF | TRBV13 | TRBD2 | TRBJ2-1 | ||
| 0.00726612 | CAVRFPRDYKLSF | TRAV21 | TRAJ20 | CASSPTGTGYYEQYF | TRBV7-9 | TRBD1 | TRBJ2-7 | ||
| 0.00635786 | CAEMEGFKTIF | TRAV5 | TRAJ9 | CASSFLVGPGGNTIYF | TRBV7-9 | − | TRBJ1-3 | ||
| 0.00544959 | CAVRDSRAAGNKLTF | TRAV1-2 | TRAJ17 | CASSKGFTSTDTQYF | TRBV6-4 | − | TRBJ2-3 | ||
| 0.00544959 | CAVRRGGSYIPTF | TRAV21 | TRAJ6 | CAGSGIQGELFF | TRBV19 | TRBD1 | TRBJ2-2 | ||
| 0.00454133 | CALSGINTDKLIF | TRAV16 | TRAJ34 | CASSPKWDGQETQYF | TRBV3-1 | TRBD2 | TRBJ2-5 | ||
| 0.00454133 | CAPLGGAGSYQLTF | TRAV21 | TRAJ28 | CASSPRQGNTGELFF | TRBV7-9 | TRBD1 | TRBJ2-2 | ||
| 0.00454133 | CIVRSLINYGQNFVF | TRAV26-1 | TRAJ26 | CASSSVSYEQYF | TRBV7-9 | − | TRBJ2-7 | ||
| NS1 reactive memory CD8+ | 120 | 0.173516 | CAGRGAGSYQLTF | TRAV21 | TRAJ28 | CASSLLSYEQYF | TRBV7-9 | − | TRBJ2-7 |
| 0.036530 | CAPLGGAGSYQLTF | TRAV21 | TRAJ28 | CASSPRQGNTGELFF | TRBV7-9 | TRBD1 | TRBJ2-2 | ||
| 0.036530 | CAGVTGGSYIPTF | TRAV27 | TRAJ6 | CASSGRAHYGYTF | TRBV7-9 | TRBD1 | TRBJ1-2 | ||
| 0.036530 | CAVGGYNKLIF | TRAV3 | TRAJ4 | CSARASTVPLYEQYF | TRBV20-1 | TRBD1 | TRBJ2-7 | ||
| 0.027397 | CAMREGNTGGFKTIF | TRAV14DV4 | TRAJ9 | CAISESQDNQPQHF | TRBV10-3 | TRBD1 | TRBJ1-5 | ||
| 0.022831 | CAVRGRGDYKLSF | TRAV1-2 | TRAJ20 | CASSSAGTLNTGELFF | TRBV7-9 | TRBD1 | TRBJ2-2 | ||
| 0.022831 | CARSYNTDKLIF | TRAV19 | TRAJ34 | CASSLEIEAFF | TRBV7-9 | − | TRBJ1-1 | ||
| 0.022831 | CAVRRGGSYIPTF | TRAV21 | TRAJ6 | CAGSGIQGELFF | TRBV19 | TRBD1 | TRBJ2-2 | ||
| 0.018265 | CAEMEGFKTIF | TRAV5 | TRAJ9 | CASSFLVGPGGNTIYF | TRBV7-9 | − | TRBJ1-3 | ||
| 0.013699 | CAMIGNTPLVF | TRAV14DV4 | TRAJ29 | CASSGLEAEQFF | TRBV7-9 | − | TRBJ2-1 | ||
| CD8+ NS3 reactive memory CD8+ | 120 | 0.09438776 | CAVQAQGYSTLTF | TRAV20 | TRAJ11 | CASSELDNEQFF | TRBV2 | − | TRBJ2-1 |
| 0.07908163 | CAVGSPLYSGGGADGLTF | TRAV8-3 | TRAJ45 | CASSLAGGYEQYF | TRBV5-6 | TRBD2 | TRBJ2-7 | ||
| 0.05102041 | CAVQAGGYSTLTF | TRAV20 | TRAJ11 | CASAEADNEQFF | TRBV13 | TRBD2 | TRBJ2-1 | ||
| 0.04336735 | CAVNGGVGNQFYF | TRAV12-2 | TRAJ49 | CSTGTPGQPLSYEQYF | TRBV20-1 | TRBD1 | TRBJ2-7 | ||
| 0.03826531 | CAASWYSGGGADGLTF | TRAV29DV5 | TRAJ45 | CASSQSDRSTYNEQFF | TRBV4-3 | − | TRBJ2-1 | ||
| 0.02806122 | CAGTGGADGLTF | TRAV12-2 | TRAJ45 | CASNPGSPEAFF | TRBV2 | TRBD2 | TRBJ1-1 | ||
| 0.02806122 | CAADGGSQGNLIF | TRAV21 | TRAJ42 | CASSEWIGTEAFF | TRBV6-1 | − | TRBJ1-1 | ||
| 0.0255102 | CAVLNSGYSTLTF | TRAV21 | TRAJ11 | CATSGVGDRGFDNEQFF | TRBV24-1 | TRBD1 | TRBJ2-1 | ||
| 0.0255102 | CALDIMDSSYKLIF | TRAV6 | TRAJ12 | CATSDLGLPEETQYF | TRBV24-1 | TRBD2 | TRBJ2-5 | ||
| 0.01785714 | CAVDNNNARLMF | TRAV39 | TRAJ31 | CASSPRFGDTYEQYF | TRBV11-3 | TRBD1 | TRBJ2-7 |