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. 2019 Aug 14;9:11802. doi: 10.1038/s41598-019-48092-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western Blot analysis detected expression of cdk10 and ETS2 in HCEC: (A) The expression level of cdk10 and ETS2 at active hours of migration (18 hours) related to non-migrating sample with beta- Actin as loading control. lane 1 NM, non-migrating; lane 2, migrating at 18 hrs. (B) Quantification and intensity measurement of relative protein expression were analyzed by Quantity One software (Bio-Rad, USA). Histograms are generated using GraphPad Prism software (7.04).Values are expressed as ± SD, significance (P < 0.005) was calculated using one way ANOVA test statistically.