FIGURE 10.

Generation of Cdk13^tm1d mice. (A) Scheme of Cdk13^tm1d allele harbors deletion of critical exons 3 and 4, causing non-functional allele of Cdk13 gene. (B) Protein extract from the brain was prepared from E14.5 embryos and protein levels of CDK13 and γ-catenin (CTNNG) were evaluated by Western blotting. No residual expression of CDK13 was observed in Cdk13^tm1d/tm1d embryos. Any significant downregulation of Ser2 and Ser5 phosphorylation was not observed in Cdk13^tm1d/tm1d mice. (C) Cdk13^tm1d/tm1d embryos exhibited distinct craniofacial phenotype with midfacial hypoplasia in comparison to wild-type embryos. Scale bar = 0.3 mm.