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. 2019 Aug 7;7:155. doi: 10.3389/fcell.2019.00155

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Copyright © 2019 Nováková, Hampl, Vrábel, Procházka, Petrezselyová, Procházková, Sedláček, Kavková, Zikmund, Kaiser, Juan, Fann, Buchtová and Kohoutek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MicroCT analysis of wild-type and Cdk13^tm1a/tm1a embryos. High-contrast differentiation resolution by X-ray computed microtomography, where Lugol’s staining was used to visualize the soft tissues. Sagittal sections through body midline (A,B) and more lateral plane at E15.5 (C,D). Horizontal sections through lung (E,F) and liver (G,H). 3D reconstruction of kidney and liver in the right side view of embryo (I,J), left side view (K,L) and caudal view (M,N) with embryo outlined in gray where segmentation of serial sections was used for the liver and kidney reconstruction. (O,P) High power of 3D imaging for liver and kidney. Horizontal view (Q,R) and sagittal detailed view (S,T) on kidney and suprarenal gland. Abbreviation used for individual organs: ag, adrenal gland; d, diencephalon; h, heart; hb, hindbrain; k, kidney; l, lung; li, liver; mb, midbrain; t, tongue; te, telencephalon; s, stomach; sc, spinal cord. Scale bar = 1 mm.