Figure 6. A strong terminator sequence compensates for lack of Sen1.
- Sequence of the engineered strong terminator (arg10‐23T) at the SPCTRNAARG.10 gene.
- ChIP‐qPCR analysis of Rpc37 around SPCTRNAARG.10 gene in the strong terminator mutant (mean ± SD from four biological replicates).
- Strand‐specific RT–qPCR was used to quantify read‐through transcripts at SPCTRNAARG.10 (arg.10) and SPCTRNASER.09 (ser.09) in the strong terminator mutant (mean ± SD from four biological replicates; P‐value obtained using the Wilcoxon–Mann–Whitney statistical test).