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. 2019 Jul 4;38(16):e99266. doi: 10.15252/embj.201899266

Figure EV1. Expression of Rab5ACA, Rab22ACA, and Rab7ADN restricts the degradation of phagocytosed antigen.

Figure EV1

  1. Phagocytosed latex beads covalently conjugated with Alexa 647‐OVA were extracted from BMDC expressing GFP or GFP‐Rab5ACA or GFP‐Rab22ACA or Rab7ADN at 1‐h intervals, and analyzed for the loss of Alexa 647 fluorescence by flow cytometry.
  2. The mean Alexa 647 fluorescence on the beads normalized to the fluorescence at the 1‐h time point is plotted (n = 3).
Data representation: In (A), a representative of three independent experiments is shown. In (B), the mean (±SEM) is plotted and analyzed by non‐linear one‐phase decay curve. The t½ obtained from the above analysis was compared. *P < 0.05. **P < 0.01, and ***P < 0.005 (one‐way ANOVA).