Figure 2. Expression of Rab5ACA, Rab22ACA, and Rab7ADN, respectively, increases hβ₂m‐enhanced TAP‐independent cross‐presentation.

• A–C
  Surface H2‐K^b levels of wild‐type and TAP1^−/− BMDC co‐expressing Rab mutants and hβ₂m were analyzed by flow cytometry.
• D–F
  Cross‐presentation of OVA by wild‐type and TAP1^−/− BMDC co‐expressing Rab mutants and hβ₂m was analyzed, and IL‐2 release is shown (D–F).

Data information: In (D–F), representative means (±SD) of at the least three independent experiments per Rab mutants setup in triplicate are plotted. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001, and “ns” is not significant (Student's t‐test).